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Yarrowia lipolytica - Biotechnological Applications | Gerold Barth | Springer
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Cotta , 6 Badal C. Saha , 6 and Stephen R. Hughes 1. Marjorie A. Elby J. Rebecca J. Sookie S. Bryan R. Michael A. Loren B. Cletus P. Kenneth M.
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Joseph O. Badal C. Stephen R. Author information Article notes Copyright and License information Disclaimer. Hughes, Phone: , Email: vog. Corresponding author. This article has been cited by other articles in PMC. Abstract Increased interest in sustainable production of renewable diesel and other valuable bioproducts is redoubling efforts to improve economic feasibility of microbial-based oil production.
Keywords: Yarrowia lipolytica UV-C mutagenesis, Yarrowia protein utilization, Acylglycerols from oleaginous yeast, Yarrowia ammonia production, Yarrowia carbohydrate substrate utilization. Introduction One of the major challenges facing commercial production of biofuels and bioproducts is cost-effective utilization, detoxification, and processing of biomass and other inexpensive carbon sources such as coffee and fruit processing wastes and other agricultural and food waste. Irradiation of Y. Open in a separate window.
Indicator for ammonia production Bromothymol blue also known as bromthymol blue; BTB is a commonly used pH indicator and was selected for ammonia screening because of its easily observed color change from yellow to blue at pH 7. Scanning electron micrographs Cells from Y.
Light microscopy Y. Ammonia quantitation assay A Megazyme Ammonia Assay kit Megazyme International, Ireland was used to determine the amount of ammonia produced by each strain. Determination of fatty acid composition of oil Fatty acid composition was determined by derivatization of oil with methanolic KOH to generate fatty acid methyl esters FAMEs as described in Ichihara et al.
Morphology Scanning electron micrographs and light microscopy photographs Figs. Oil production The wild-type and mutant strains were also evaluated for oil triglycerides production using Sudan Black B indicator. Table 2 Fatty acid composition of lipids produced using Yarrowia lipolytica mutant strain F on Kluyveromyces -treated coffee waste compared to that for other Y.
Substrate utilization In plate assays, the mutant strains grew on a variety of substrates similar to the wild-type strain as shown in Fig. Morphological evaluation Y. Evaluation of doubling times Madzak et al. Comparison of ammonia production, protein levels, and 2-PE production Y. Oil production evaluation The complexity and multiplicity of genes present in the genome of Y.
Substrate utilization by wild-type and mutant strains Currently, utilization of cellulosic biomass including agricultural and food wastes has drawn increasing attention as a means of minimizing the cost of bio-oil production Tsigie et al. Acknowledgments We gratefully acknowledge the financial support of the Organization of American States for the research on utilization of coffee waste.
Conflict of interest The authors declare that they have no competing interest. Footnotes Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. References Abghari A, Chen S. Yarrowia lipolytica as an oleaginous cell factory platform for production of fatty acid-based biofuels and bioproducts.
Front Energy Res. Physical properties and heavy metal uptake of encapsulated Escherichia coli expressing a metal binding gene NCP J Microencapsul. Yarrowia lipolytica. In: Wolf K, editor. Nonconventional yeasts in biotechnology. Berlin: Springer; Physiology and genetics of the dimorphic fungus Yarrowia lipolytica.
Yarrowia lipolytica as a model for bio-oil production.
Prog Lipid Res. Physical treatment of high EPA Yarrowia lipolytica biomass increases the availability of n-3 highly unsaturated fatty acids when fed to Atlantic salmon. Aquac Nutr. Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production.
Nat Commun. A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica. J Microbiol Methods. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. Effect of salts on the co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae. Biotechnol Biofuels. Yarrowia lipolytica : the novel and promising 2-phenylethanol producer. J Ind Microbiol Biotechnol.
Genome evolution in yeasts. Chemical composition of coffee-berry by-products. Coffee pulp; composition, technology, and utilization. Screening of yeasts for the production of the aroma compound 2-phenylethanol in a molasses-based medium. Biotechnol Lett. Organic acid production by the yeast Yarrowia lipolytica : a review of prospects. Appl Biochem Microbiol.
Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica. Bioresour Technol. Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering.
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A survey of yeasts in traditional sausages of southern Italy. Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica. Microb Cell Factories. Yarrowia lipolytica and its multiple applications in the biotechnological industry. Sci World J. Yarrowia lipolytica : safety assessment of an oleaginous yeast with a great industrial potential. Crit Rev Microbiol.
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SpringerBriefs in Microbiology. Springer International Publishing. Single cell oil production from low-cost substrates: the possibility and potential of its industrialization. Biotechnol Adv. Integrated automation for continuous high-throughput synthetic chromosome assembly and transformation to identify improved yeast strains for industrial production of biofuel and bio-based chemicals. Genetic transformation systems in fungi. Heidelberg: Springer; Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell.
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Rational and evolutionary engineering approaches uncover a small set of genetic changes efficient for rapid xylose fermentation in Saccharomyces cerevisiae. Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Process Technol. Valorization of industrial waste and by-product streams via fermentation for the production of chemicals and biopolymers.
Chem Soc Rev. The yeasts: a taxonomic study. London: Elsevier; Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria. Heterologous protein expression and secretion in Yarrowia lipolytica. In: Barth G, editor. Yarrowia lipolytica , microbiology monographs.
Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica : a review. J Biotechnol. Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica. Lactate and amino acid catabolism in the cheese-ripening yeast Yarrowia lipolytica. Transcription analysis of recombinant industrial and laboratory Saccharomyces cerevisiae strains reveals the molecular basis for fermentation of glucose and xylose. Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica.
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